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Image Search Results
Journal: Biophysical chemistry
Article Title: Binding specificity of the ectodomain of the parathyroid hormone receptor.
doi: 10.1016/j.bpc.2011.01.002
Figure Lengend Snippet: Fig. 3. Isothermal titration calorimetry during PTH to nPTH1R binding. A solution of (A) 348 μM PTH (1–37), (B) 350 μM PTH (13–34) and (C) 350 μM PTH (1–34) V21Y was titrated stepwise to a solution of 27 μM nPTH1R in 50 mM Tris–HCl, pH 8 at 293.15 K. Peak areas from every injection step were integrated and analyzed with the ORIGIN software (MicroCal Software, Northampton, MA, USA). The solid line represents a fit according to the ‘one set of sites’ binding model. The experiments were carried out in triplicate (Table 1).
Article Snippet: The peak areas produced by every injection were integrated and analyzed with the
Techniques: Isothermal Titration Calorimetry, Binding Assay, Injection, Software